Semen Analysis (by LabCE)

1.5 P.A.C.E. contact hour(s)

(based on 526 customer ratings)

Author: Judy Stern, PhD
Reviewer: Barbara Cebulski, MS, MLS(ASCP)

Course provided by LabCE.

This course details all the steps that are necessary to ensure an accurate sperm count. Many illustrations and descriptions are provided to acquaint you with normal and abnormal morphology. This course is an excellent source of continuing education for medical technologists and technicians. It is also appropriate for training laboratory personnel, cross training, and clinical laboratory science students.

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Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-060-22, approved through 7/31/2024
Course number 20-925649, approved through 7/31/2024


  • Identify the preanalytic procedures that must be followed to ensure reliable semen analysis results.
  • List the requirements for collection, transport, and accession of a specimen for semen analysis.
  • Identify the characteristics of semen that should be noted during the macroscopic examination of the sample.
  • Explain how to perform a sperm count.
  • Discuss the method for determining and reporting sperm motility according to the current World Health Organization (WHO) guidelines.
  • Identify sperm morphologies according to current WHO guidelines.

Customer Ratings

(based on 526 customer ratings)

Course Outline

  • Introduction to Semen Analysis
      • Composition of Semen
      • Purpose of Semen Analysis
      • Limits of Semen Analysis
      • Safety Precautions
  • Specimen Collection, Transportation, and Accessioning
      • Specimen Identification
      • Collection
      • Collection, continued
      • Documenting Collection Information
  • Macroscopic Examination of Semen
      • Beginning the Analysis
      • Liquefaction
      • Appearance of Semen
      • Volume
      • Viscosity
      • pH
  • Initial Microscopic Examination and Assessment of Sperm Motility
      • Initial Microscopic Examination of Semen
      • Initial Microscopic Examination of Semen, continued
      • Motility: Standardization of Assessment Method
      • Assessing Motility
      • Assessing Motility: Example of Non-progressive Motility
      • Wet Mount Assessment of Sperm Motility
      • Automated Procedures
      • Viability
  • Sperm Count
      • Sperm Concentration and Sperm Count
      • Sperm Counting Methods
      • Improved Neubauer Hemocytometer
      • Diluting a Specimen for Counting on a Hemocytometer
      • Loading the Hemocytometer and Performing the Count
      • Criteria for Sperm Counts Using a Hemocytometer
      • Other Counting Chambers
      • Assessment of Post-Vasectomy and Azoospermic Specimens
  • Non-Sperm Cells in Semen
      • Other Cells in Semen: Epithelial cells
      • Other Cells in Semen: Round Cells and Red Blood Cells (RBCs)
      • Other Cells in Semen: Round Cells and RBCs, continued
      • Round Cells in Semen
  • Morphology of Sperm
      • Morphology of Sperm
      • Preparing Semen Smears for Evaluation of Sperm Morphology
      • Evaluating Sperm Morphology
      • Abnormal Forms
      • Abnormal Heads
      • Abnormal Tails
      • Normal Head Shape
      • Sperm with Enlarged Head and Split Tail
      • Double-Headed Sperm
      • Pinhead
      • Two Abnormalities
      • Tapered Head
      • Constricted Head
  • Reference Values and Course References
      • Summary: Reference Values
      • References

Additional Information

Level of Instruction: Basic

Intended Audience: Clinical laboratory science students, and medical technologists and technicians seeking review, cross training, or continuing education opportunities. It is also appropriate for medical students and pathology residents.

Author information: Judy Stern, PhD is a Professor of Pathology, Professor of Obstetrics and Gynecology, and Director, Reproductive Sciences Laboratory, Dartmouth-Hitchcock Medical Center.  Dr. Stern holds a PhD from the University of Tennessee. She has authored and co-authored multiple publications in the field of Reproductive Technology.

Reviewer information: Barbara Cebulski, MS, MLS(ASCP) has over 40 years of experience in the medical laboratory profession as a technologist, section supervisor, and laboratory manager. She was an Inspection and Technical Specialist for nine years with the College of American Pathologists in the Laboratory Accreditation Program and, until her retirement in 2015, was Program Director for MediaLab, Inc. Barbara holds a Masters in Instructional Technology from Georgia State University.

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Course provided by LabCE.




alternate counters 2

Normal sperm round cells



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