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Course provided by LabCE.

See more courses in: Clinical Chemistry / Urinalysis / Toxicology

Continuing Education Credits

Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 1.5 hour(s)
Course number 20-563193, approved through 9/1/2020

Objectives

  • Explain the principle of electrophoresis and compare the factors that determine the mobility and rate of migration in electrophoresis.
  • Describe the role of the buffer in electrophoresis, types of support mediums, and the kinds of specimens used in electrophoresis.
  • Compare and contrast the following types of electrophoresis: routine electrophoresis, polyacrylamide gel electrophoresis, high resolution electrophoresis, capillary electrophoresis, isoelectric focusing, two-dimensional electrophoresis, pulsed field electrophoresis, and immunochemical electrophoresis.
  • Define each of the following terms: amphoteric, ampholyte(s), isoelectric point, sodium dodecyl sulfate, cathode, and anode.
  • Name and describe the stains used in electrophoresis.
  • Evaluate causes of irregular, distorted or atypical electrophoresis bands, along with correct sample application and proper care and use of buffers and support mediums.
  • Explain electroendosmosis and wick flow as encountered in electrophoresis and assess their effect on separated bands.
  • Discuss the theory behind and uses of densitometry.
  • Describe increased electrophoresis usage today and incorporation of electrophoresis techniques in molecular diagnostics.

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Course Outline

  • Basic Principals of Electrophoresis
      • Introduction
      • Principle of Electrophoresis
      • Amphoteric
      • Isoelectric Point (pI)
      • Mobility or Rate of Migration
      • Rate of Migration
  • Electrophoresis and Buffers
      • Role of Buffers
      • Buffers and pH
  • Specimens for Electrophoresis
      • Specimens
  • Electrophresis and Support Media
      • Types of Support Media
      • Agarose Gel
      • Polyacrylamide Gels
  • Electrophoresis Instrumentation
      • Electrophoresis Equipment
      • Automation
  • Types of Electrophoresis
      • Types of Electrophoresis
      • Routine Electrophoresis
      • High Resolution Electrophoresis (HRE)
      • Polyacrylamide Electrophoresis (PAGE)
      • Denaturing Polyacrylamide Gels
      • Capillary Electrophoresis (CE)
      • CE Advantages and Applications
      • Isoelectric Focusing (IEF)
      • IEF Advantages and Applications
      • Immunofixation Electrophoresis
      • Pulsed Field Electrophoresis
      • Two-Dimensional Electrophoresis
      • Two-Dimensional Electrophoresis Advantages and Applications
      • Types of Electrophoresis-- Summary
  • Visualization and Detection
      • Visualization and Detection Methods
      • Stains and Dyes
      • Densitometry
      • Labeled Probes
  • Technical Considerations and Electrophoresis Troubleshooting
      • Technical Considerations and Electrophoresis Troubleshooting Topics
      • Sample Application
      • Buffers
      • Support Media
      • Electroendosmosis
      • Wick Flow
      • Troubleshooting Irregular, Distorted, or Atypical Bands
  • Resurgence of Electrophoresis
      • Resurgence of Electrophoresis
      • Electrophoresis and Molecular Diagnostics
      • Blotting Techniques
      • Uses of CE in Molecular Diagnostics
  • References
      • References
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