Electrophoresis (by LabCE)

1.5 P.A.C.E. contact hour(s)

(based on 471 customer ratings)

Author: Mary Ellen Koenn, MS, MLS(ASCP)
Reviewer: Leslie Lovett, MS, MLS(ASCP)

Course provided by LabCE.

This course discusses the many types and applications of electrophoresis in the clinical laboratory. Commonly used terms are defined and procedures are described, including explanations of various electrophoretic patterns. A discussion on newer molecular diagnostic techniques that incorporate electrophoresis conclude this in depth look at electrophoresis.

See more courses in: Clinical Chemistry / Urinalysis / Toxicology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-002-17, approved through 1/31/2019
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 1.5 hour(s)
Course number 20-563193, approved through 9/1/2020

Objectives

  • Explain the principle of electrophoresis and compare the factors that determine the mobility and rate of migration in electrophoresis.
  • Describe the role of the buffer in electrophoresis, types of support mediums, and the kinds of specimens used in electrophoresis.
  • Compare and contrast the following types of electrophoresis: routine electrophoresis, polyacrylamide gel electrophoresis, high resolution electrophoresis, capillary electrophoresis, isoelectric focusing, two-dimensional electrophoresis, pulsed field electrophoresis, and immunochemical electrophoresis.
  • Define each of the following terms: amphoteric, ampholyte(s), isoelectric point, sodium dodecyl sulfate, cathode, and anode.
  • Name and describe the stains used in electrophoresis.
  • Evaluate causes of irregular, distorted or atypical electrophoresis bands, along with correct sample application and proper care and use of buffers and support mediums.
  • Explain electroendosmosis and wick flow as encountered in electrophoresis and assess their effect on separated bands.
  • Discuss the theory behind and uses of densitometry.
  • Describe increased electrophoresis usage today and incorporation of electrophoresis techniques in molecular diagnostics.

Customer Ratings

(based on 471 customer ratings)

Course Outline

  • Basic Principals of Electrophoresis
      • Introduction
      • Principle of Electrophoresis
      • Amphoteric
      • Isoelectric Point (pI)
      • Mobility or Rate of Migration
      • Rate of Migration
  • Electrophoresis and Buffers
      • Role of Buffers
      • Buffers and pH
  • Specimens for Electrophoresis
      • Specimens
  • Electrophresis and Support Media
      • Types of Support Media
      • Agarose Gel
      • Polyacrylamide Gels
  • Electrophoresis Instrumentation
      • Electrophoresis Equipment
      • Automation
  • Types of Electrophoresis
      • Types of Electrophoresis
      • Routine Electrophoresis
      • High Resolution Electrophoresis (HRE)
      • Polyacrylamide Electrophoresis (PAGE)
      • Denaturing Polyacrylamide Gels
      • Capillary Electrophoresis (CE)
      • CE Advantages and Applications
      • Isoelectric Focusing (IEF)
      • IEF Advantages and Applications
      • Immunofixation Electrophoresis
      • Pulsed Field Electrophoresis
      • Two-Dimensional Electrophoresis
      • Two-Dimensional Electrophoresis Advantages and Applications
      • Types of Electrophoresis-- Summary
  • Visualization and Detection
      • Visualization and Detection Methods
      • Stains and Dyes
      • Densitometry
      • Labeled Probes
  • Technical Considerations and Electrophoresis Troubleshooting
      • Technical Considerations and Electrophoresis Troubleshooting Topics
      • Sample Application
      • Buffers
      • Support Media
      • Electroendosmosis
      • Wick Flow
      • Troubleshooting Irregular, Distorted, or Atypical Bands
  • Resurgence of Electrophoresis
      • Resurgence of Electrophoresis
      • Electrophoresis and Molecular Diagnostics
      • Blotting Techniques
      • Uses of CE in Molecular Diagnostics
  • References
      • References

Additional Information

Level of instruction: Intermediate

Intended Audience: Clinical laboratory technologists and technicians, and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
 
Author information: Mary Ellen Koenn, MS, MLS(ASCP) is an associate professor emeritus, West Virginia University School of Medicine, Medical Laboratory Science Program. During her career as a medical technologist and educator, she has been a laboratory supervisor and manager and has held several teaching positions. She is the author of numerous articles for laboratory publications and textbook chapters and is a frequent presenter at laboratory seminars and workshops. Ms. Koenn holds a Master of Science degree in Medical Technology.
 
Reviewer information: Leslie Lovett, MS, MT(ASCP) is the Clinical Education Coordinator of the Medical Laboratory Technology Program and a professor at Pierpont Community and Technical College in West Virginia.  She holds a MS degree in Medical Technology with classes toward a PhD in Pharmacology and Toxicology.

Course Description: This course discusses the many types and applications of electrophoresis in the clinical laboratory. Commonly used terms are defined and procedures are described, including explanations of various electrophoretic patterns. A discussion on newer molecular diagnostic techniques that incorporate electrophoresis conclude this in depth look at electrophoresis.

How to Subscribe
MLS & MLT Comprehensive CE Package (by LabCE)
Package of 70 online courses$95
Add to cart
Single online course$25
Add to cart
  • Order for instant access through any computer, any browser.
  • No shipping, faxing, or waiting for certificates.
  • Print or save your certificate of completion as soon as you've completed the course.
  • You'll have 90 days to complete your purchased courses.
Course provided by LabCE.
Immunofixation Electrophresis


PAGE Separation of Two Similar Proteins


Isoelectric_Point


ELP final



 
ASCLS CE's design and platform are provided by MediaLab, Inc.