Zika Virus: Overview and Laboratory Testing (by LabCE)

2 P.A.C.E. contact hour(s)

(based on 861 customer ratings)

David J Moffa, PhD, BCLD
Reviewer: Rodney E. Rohde, PhD, MS, SV, SM(ASCP)CMMBCM

Course provided by LabCE.

See more courses in: Immunology / Serology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2 hour(s)
Course number 578-023-17, approved through 7/31/2019
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Serology/Immunology): 2 hour(s)
Course number 20-584775, approved through 9/1/2020

Objectives

  • Understand the virology and pathogenesis of the Zika virus.
  • Describe the modes of transmission, symptoms, and infection risks.
  • Determine the risk of Zika virus infection during pregnancy and the evaluation and consequences of congenital infections.
  • Discuss the diagnosis of Zika virus infection using a combination of clinical symptoms, history, and diagnostic testing.
  • Define the main laboratory tests used for Zika virus identification, including RT-PCR, Zika IgM ELISA and plaque reduction neutralization tests (PRNT).
  • Review the latest Centers for Disease Control and Prevention (CDC) guidelines on Zika virus testing and diagnosis, with emphasis on the evaluation of women and newborns with peripartum Zika virus exposure.
  • Examine the prevention and treatment of Zika virus infections and the status of vaccine development.
  • Review the CDC's Zika virus testing algorithms for symptomatic and asymptomatic individuals.

Customer Ratings

(based on 861 customer ratings)

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Course Outline

  • Introduction: Virology and Transmission
      • Virology and Transmission
      • Virology and Transmission (continued)
  • History
      • Transmission History
  • Symptoms, Infection, and Risks
      • Symptoms of Zika Infection
      • Clinical Manifestation and Congenital Zika Virus Infection
      • Zika Virus Infection Risks
  • Patient Management: General population, Pregnancy, and Newborns
      • Zika Management for the General Population
      • Zika Virus Infection Management for Pregnant Women
      • Zika Virus Infection Management for Women Wanting to Become Pregnant
      • Zika Virus Infection Management for Women Wanting to Prevent Pregnancy
      • Newborn Management, Evaluation, and Follow-up
  • Diagnosis and Laboratory Testing
      • Overview: Diagnosis and Testing
      • Overview: Diagnosis and Testing (continued)
      • Specimen Types and Collection
    • Laboratory Tests Used for Zika Virus Identification
      • Zika Virus Testing Information Available for Laboratories
      • CDC-Approved Zika Assays and Qualified Laboratories
      • Information for Laboratories: Diagnostic Tests and Emergency Use Authorization (EUA)
      • Real-Time Reverse Transcription-Polymerase Chain Reaction (rRT-PCR)
      • Trioplex Real-Time RT-PCR Assay
      • Zika IgM ELISA (Zika MAC-ELISA)
      • Zika MAC-ELISA: Key Facts
      • Plaque Reduction Neutralization Tests (PRNT)
      • Key Considerations: Interpreting and Reporting Zika Test Results
    • CDC Guidelines on Zika Virus Testing and Diagnosis
      • Overview and Testing Algorithms: Pregnant or Non-Pregnant Symptomatic Individuals
      • Overview and Testing Algorithm: Asymptomatic Pregnant Women
  • Prevention, Treatment and Vaccine Development
      • Prevention
      • Treatment
      • Vaccine Development
  • Summary and Recommendations
      • Summary and Recommendations:
      • Summary and Recommendations (continued)
      • Links to CDC and FDA Zika Information Updates
  • References
      • References

Additional Information

Level of instruction: Intermediate

Intended Audience: Clinical laboratory professionals including medical laboratory scientists, medical technologists, and technicians. This course is also appropriate for clinical laboratory science and MLT students, pathologists, and clinicians. 
Author information: David J. Moffa, PhD, BCLD, has over 30 years of experience in the healthcare industry as an executive manager, clinical laboratory director, and medical laboratory scientist. He is currently a technical consultant for Kentmere Healthcare, Wilmington, DE, and until his retirement, was the Regional Director for LabCorp, Inc. He holds a PhD in medical biochemistry from the School of Medicine, West Virginia University.
Reviewer information: Rodney E. Rohde, PhD, MS, SV, SM(ASCP)CMMBCM is the Professor and Chair of the Clinical Laboratory Science Program in the College of Health Professions at Texas State Universit in San Marcos, TX. Dr. Rohde brings his career experiences as a public and clinical microbiologist and infectious disease expert from CDC and the Texas Department of State Health Services Bureau of Laboratories and Zoonosis Control to the classroom. He is also the Associate Dean for Research and has been recognized with multiple research and teaching awards of excellence, including being named one of the Top 20 Professors of Clinical Laboratory Science You Should Know and the 2015 urEssential award from Cardinal Health.  
Course Description: This course is intended to provide a detailed description of the virology and pathogenesis of the Zika virus, including the modes of transmission, symptoms, and infection risks. The primary laboratory tests used for Zika virus identification are included. The CDC's Zika virus testing algorithms for symptomatic and asymptomatic individuals are reviewed, emphasizing the evaluation of women and newborns with peripartum Zika virus exposure. Finally, the prevention and treatment of Zika virus infections and the status of vaccine development is examined. 

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Course provided by LabCE.
Overview of RT-PCR

(Note:Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Tex


<em>Aedes aegypti</em> deposited eggs. Image courtesy CDC.


<i>Aedes aegypti</i>. Image courtesy of the CDC.


Overview of the PCR
(Taken from Government public domain use from the National Library of Medicine (NLM). Source: PCR Introduction, NCBI, NIH, available at 
http://www.ncbi.nlm.nih.gov/genome/probe/doc/TechPCR.shtml



 
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