Anaerobes - It doesn't have to be Rocket Science! (by ASCLS)

This course does not carry any current continuing education credits.

(based on 16 customer ratings)

Robert C. Fader, PhD, D(ABMM)

Course provided by ASCLS.

This self-study examines the pre-analytic, analytic, and post-analytic stages as they relate to:
•The tools to rapidly identify anaerobic organisms.
•Providing clinicians with actionable information concerning the presence of an anaerobe in a specimen which will improve patient care.
•Susceptibility testing rapid methods for anaerobe identification outlined in the CLSI M35-A2

See more courses in: Microbiology


  • Define the significance of anaerobic infections
  • Describe proper methods of specimen collection, transport and processing
  • List the rapid identification methods for commonly encountered anaerobes
  • Describe when to perform susceptibility testing of anaerobes
  • Identify the correct organism given the Gram stain, colony morphology, media growth, and rapid test results.

Customer Ratings

(based on 16 customer ratings)

Course Outline

  • PowerPoint Slides
      • Anaerobes: It doesn't have to be Rocket Science!
      • Course Overview
      • Goal for Course
      • Author
      • Table of Contents
      • Learning Objectives
      • Section I: What is an anaerobe? (Pre-Analytic Process)
      • Definition of an anaerobe
      • What makes an organism an anaerobe?
      • Infections of endogenous origin
      • Infections of exogenous origin
      • Specimen collection and transport
      • Transport tubes
      • Unacceptable specimens
      • Anaerobic Media
      • Selective Anaerobic Media
      • Selective Anaerobic Media, cont.
      • Anaerobic Broth Media
      • Anaerobic Culture Methods
      • Section II: Anaerobic Identification (Analytic Process)
      • When to examine plates
      • Identification Using Rapid Methods
      • Anaerobic Culture
      • Antibiotic Disks for Presumptive ID
      • Special Potency Antibiotic Disks
      • Rapid Tests
      • CLSI M35-A2
      • Section III: Gram Negative Anaerobes
      • Bacteroides fragilis group
      • Bacteroides fragilis group - Presumptive ID
      • Prevotella/Porphyromonas
      • Prevotella - Presumptive ID
      • Porphyromonas sp.- Presumptive ID
      • Bilophila wadsworthia
      • Bilophila wadsworthia - Presumptive ID
      • Fusobacterium nucleatum
      • Fusobacterium nucleatum - Presumptive ID
      • Campylobacter (Bacteroides) ureolyticus
      • Campylobacter (Bacteroides) ureolyticus - Presumptive ID
      • Veillonella
      • Veillonella - Presumptive ID
      • Gram Stain of Veillonella
      • Section IV: Gram Positive Anaerobes
      • Clostridium perfringens
      • C. perfringens - Presumptive ID
      • C. perfringens Gram Stain
      • Clostridium difficile
      • C. difficile - Presumptive ID
      • C. difficile Gram Stain
      • Clostridium septicum
      • C. septicum - Presumptive ID
      • C. septicum Gram Stain
      • Clostridium sordellii
      • C. sordellii - Presumptive ID
      • C. sordellii Gram Stain
      • Clostridium tetani - Tetanus
      • C. tetani - Presumptive ID
      • The "Peptostreptococci"
      • Peptostreptococcus - Presumptive ID
      • "Peptostreptococcus"Gram Stain
      • Propionibacterium acnes
      • P. acnes - Presumptive ID
      • Propionibacterium Gram Stain
      • Section V: Susceptibility Testing (Post-Analytic Comments)
      • Susceptibility Testing
      • Susceptibility Comments
      • Susceptibility Comments, cont.
      • Conclusion / Questions

Additional Information

A great deal of time and expense can be wasted on over-working anaerobe cultures. Providing clinicians with actionable information concerning the presence of anaerobes in a specimen can and will improve patient care. This self-study will focus on rapid methods for anaerobe identification outlined in the CLSI M35-A2 - Abbreviated Identification of Bacteria and Yeast.

Intermediate level of difficulty

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Course provided by ASCLS.
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