MALDI-TOF in the Clinical Microbiology Laboratory (by ASCLS)

1.5 P.A.C.E. contact hour(s)

(based on 21 customer ratings)

Christopher Doern, PhD, D(ABMM), Associate Director of Clinical Microbiology, Virginia Commonwealth University Medical Center

Course provided by ASCLS.

This self-study will address several important aspects of instituting MALDI-TOF MS for organism identification. One strategy for justifying the significant cost of the instrument will be provided. It will also briefly define: (1) some things that should be considered when deciding which platform to purchase, (2) how to validate MALDI-TOF MS for clinical use and (3) the implementation of MALDI-TOF MS for clinical care. This self-study will conclude with a discussion of work-flow as well as an analysis of the post-implementation impact on patient care.

See more courses in: Microbiology

Included In These Course Packages

Continuing Education Credits

Continuing Education Credit Hour(s): 1.5 hour(s)
Course number 108-293-16, approved through 12/31/2018
P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification) (CACMLE): 1.5 hour(s)
Course number 108-293-16, approved through 12/31/2018
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Microbiology/Mycology/Parasitology): 1.5 hour(s)
Course number 20-521331, approved through 12/31/2018

Objectives

  • Describe the cost justification of MALDI-TOF MS.
  • Define the process of validation of MADLI-TOF MS for organism identification.
  • Analyze work-flow and the impact MALDI-TOF MS has on testing turn-around time.

Customer Ratings

(based on 21 customer ratings)

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Course Outline

  • PowerPoint Slides
      • MALDI-TOF MS in the Microbiology Laboratory
      • Disclosures
      • Learning Objectives
      • What does it cost?
      • Can we afford MALDI-TOF MS?
      • Generic Cost Analysis
      • Proposal for Capital Purchase
      • Our Capital Purchase Proposal
      • Background: Current Methods vs. MALDI-TOF
      • Current conditions: Current Methods vs. MALDI-TOF
      • Goal of the Purchase
      • Analysis: Cost Evaluation
      • Analysis: Cost Evaluation
      • Available Platforms
      • Bruker: MALDI-TOF Procedure
      • Bruker: MALDI-TOF Procedure, cont.
      • Bruker: MALDI-TOF Procedure, cont.
      • Bruker: MALDI-TOF Procedure, cont.
      • Bruker: MALDI-TOF Procedure, cont.
      • bioMerieux: MALDI-TOF Procedure
      • bioMerieux: MALDI-TOF Procedure, cont.
      • bioMerieux: MALDI-TOF Procedure, cont.
      • bioMerieux: MALDI-TOF Procedure, cont.
      • Discussion: Bruker vs. bioMerieux
      • How do you validate the instrument?
      • Validation Approach of 6 US Laboratories
      • Important aspects of validation
      • Things you will need to validate
      • Spotting Method: Technique matters
      • Database Updates
      • Validation Strategy
      • VCU Approach
      • Database Revalidation Results Summary
      • What about Verifying an DFA-Approved Database?
      • What about Verifying an DFA-Approved Database?, cont.
      • What about work flow?
      • Implementation of MALDI into Clinical Use
      • Reporting considerations
      • Which one is not part of the E. cloacae complex?
      • What about work flow?
      • Pre-MALDI Work Flow - Hospital #1
      • Post-MALDI Work Flow - Hospital #1
      • Post-MALDI Work Flow - Hospital #2
      • Analysis - MALDI Work Flow
      • The Work Flow of 2 Different Culture Types
      • Cystic Fibrosis Culture Work Up
      • Blood Culture Work Up
      • Blood Culture Work Flow
      • Hospital #1 vs. Hospital #2 - Post MALDI BC Data
      • Will Faster Results Equal Better Care for Patients?
      • The End

Additional Information

This self-study will address several important aspects of instituting MALDI-TOF MS for organism identification. One strategy for justifying the significant cost of the instrument will be provided. It will also briefly define: (1) some things that should be considered when deciding which platform to purchase, (2) how to validate MALDI-TOF MS for clinical use and (3) the implementation of MALDI-TOF MS for clinical care. This self-study will conclude with a discussion of work-flow as well as an analysis of the post-implementation impact on patient care.

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Microbiology Course Package (10 hours) (by ASCLS)
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Course provided by ASCLS.
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