Laboratory Diagnosis of Cystic Fibrosis (by LabCE)

1 P.A.C.E. contact hour(s)

(based on 314 customer ratings)

Author: Vicky LeGrys, DA, MT(ASCP)
Reviewer: Rebecca J. Laudicina, PhD, MLS(ASCP)CM

Course provided by LabCE.

This course discusses the clinical presentation of cystic fibrosis (CF) and how genetic mutations can lead to the manifestation of the disorder. Laboratory testing used in the diagnosis of CF is presented including the components of a quantitative sweat chloride test. Finally, current treatment options for the disease are discussed.

See more courses in: Clinical Chemistry / Urinalysis / Toxicology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-033-21, approved through 6/30/2023
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 1 hour(s)
Course number 20-867432, approved through 6/30/2023


  • Describe the clinical presentation of cystic fibrosis (CF).
  • Describe how mutations in the CF gene lead to the clinical manifestations of the disorder.
  • Outline the laboratory diagnosis of CF to include newborn screening, the sweat test, and DNA testing.
  • Describe the components of a quantitative sweat chloride test to include reference ranges.
  • Compare screening sweat tests to confirmatory sweat tests.
  • Discuss the current treatment options for CF.

Customer Ratings

(based on 314 customer ratings)

Users Also Bought

1 P.A.C.E.® contact hour
(1,401 ratings) $20 Add to cart
13.5 P.A.C.E.® contact hours
(3 ratings) $44 Add to cart
1.5 P.A.C.E.® contact hours
(414 ratings) $20 Add to cart

Course Outline

  • Introduction to Cystic Fibrosis
      • Course Introduction
  • The History of CF
      • History of CF and Diagnostic Testing
  • Pathophysiology of CF
      • Pathophysiology of CF
      • Mutations of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
  • Clinical Presentation
      • Overview of Clinical Characteristics
      • Pulmonary Disease
      • Pancreatic Disease
      • Sweat Gland Involvement
      • Gastrointestinal and Liver Disease
      • Reproductive System
  • Laboratory Diagnosis of CF
      • Laboratory Diagnosis of CF
      • Newborn Screening (NBS) in the United States
      • DNA Testing
    • Sweat Testing
      • Sweat Testing
      • Sweat Stimulation
      • Sweat Collection
      • Collecting An Adequate Sweat Sample
      • Quantitative Sweat Analysis
      • Screening Tests for Sweat
      • Reference Intervals for Sweat Chloride
      • Reference Intervals for Sweat Conductivity
      • Quality Assurance for Sweat Testing
  • Treatment
      • Treatment
      • Treating the Symptoms
      • Treating the Protein Defect
      • Gene Replacement
  • Summary
      • Summary
  • References
      • References

Additional Information

Level of instruction: Intermediate

Intended Audience: Medical laboratory scientists, medical technologists, and technicians. This course is also appropriate for medical laboratory science students and pathology residents.

Author information: Vicky LeGrys, DA, MT(ASCP) is a professor in the Division of Clinical Laboratory Science in the School of Medicine at the University of North Carolina Chapel Hill where she is responsible for the lecture and laboratory courses in Biochemistry, Clinical Chemistry, and Quality Assurance. She holds a Doctorate of Arts and a Master of Science in Medical Technology from Catholic University of America, Washington DC with a major in Clinical Chemistry.
Reviewer information: Rebecca J. Laudicina, PhD, MLS(ASCP)CM is Professor Emeritus in the Division of Clinical Laboratory Science at The University of North Carolina at Chapel Hill’s School of Medicine. She obtained a Master of Education and PhD in Educational Psychology from Temple University. She is the author of numerous hematology-related publications.

How to Subscribe
MLS & MLT Comprehensive CE Package (by LabCE)
Package of 106 online courses$95
Add to cart
Single online course$20
Add to cart
  • Order for instant access through any computer, any browser.
  • No shipping, faxing, or waiting for certificates.
  • Print or save your certificate of completion as soon as you've completed the course.
  • You'll have 90 days to complete your purchased courses.
Course provided by LabCE.
Sweat gland Normal CF

clinical characteristics

CFTR mutant

collection of sweat into macroduct_edit

digital chloridometer_edit

ASCLS CE's design and platform are provided by MediaLab, Inc.