The Histology of Dermatological Specimens - Part 2 (by LabCE)

1 P.A.C.E. contact hour(s)

(based on 91 customer ratings)

Author: Diana Harrington, BS, HT(ASCP)
Reviewer: Brooke Eguia, BS, MS, HTL(ASCP)

Course provided by LabCE.

This course, the second in the dermatological specimens series, addresses the most common concerns of working with dermatology specimens and provides tips to create high quality slides for the dermatopathologist. Explored topics include optimal processing, sectioning, and recut protocols for skin biopsies, shaves, and excisions. The histomorphology of skin is reviewed and commonly used H&E and special stains are discussed. Possible sources of problems are also investigated and solutions are applied to prevent artifacts on skin sample slides.

See more courses in: Histology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-016-17, approved through 4/30/2019
Course number 20-547949, approved through 9/1/2020

Objectives

  • Describe the optimal processing protocol for skin biopsies and fatty skin excisions, including how fat content and tissue size affect processing.
  • Explain why accurate embedding of skin samples is critical to diagnosis and describe the best way to embed skin to obtain high quality slides.
  • Describe the most common sectioning and recutting protocols for shaves, punches, and excisions.
  • Identify sectioning artifacts, their possible sources, and solutions.
  • Identity the skin layers and cells of a properly stained hematoxylin and eosin (H&E) slide.
  • Identify the most commonly used special stains for skin specimens and the cells and structures they highlight.

Customer Ratings

(based on 91 customer ratings)

Course Outline

  • Introduction to Skin Histology
      • Introduction to Skin Histology
  • Review of Skin Histomorphology
      • The Importance of Understanding Skin Morphology
      • Overview of Skin Layers
  • Skin Sample Identification
      • Identifying Skin Sample Types
  • Skin Paraffin Processing
      • Paraffin Processing of Formalin-Fixed Skin Samples
      • Reviewing the Processing Steps
      • Choosing Skin Processing Schedules
  • Skin Embedding Techniques
      • Embedding Skin Tissue
      • General Embedding Considerations
      • Skin Embedding Techniques
  • Skin Sectioning Techniques
      • Sectioning Skin Tissue
      • Skin Sectioning Techniques
      • Skin Sectioning Protocols
      • Skin Sectioning Artifacts
      • Skin Sectioning Troubleshooting
  • Skin Staining - H&E and Special Stains
      • Hematoxylin and Eosin (H&E) Stained Skin
      • Identifying Skin Cells and Structures in Hematoxylin and Eosin (H&E) Stain
      • Special Stains for Skin
      • Special Stains for Skin: Fungi
      • Special Stains for Skin: Bacteria
      • Special Stains for Skin: Spirochetes
      • Special Stains for Skin: Melanin and Amyloid
  • Conclusion to Skin Histology
      • Conclusion
  • References
      • References

Additional Information

Level of instruction: Basic 
 
Intended audience:  Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.  
 
Author information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
 
Reviewer information: Brooke Eguia, BS, MS, HTL(ASCP) is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology and from Capella University with a Master of Science in Human Service with a specialization in Health Care Administration. During her time as an undergraduate, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. Her senior year, she co-authored for aquatic toxicology research that Aquaculture published in January 2009. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College, as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.
 
Course description: This course, the second in the dermatological specimens series, addresses the most common concerns of working with dermatology specimens and provides tips to create high quality slides for the dermatopathologist. Explored topics include optimal processing, sectioning, and recut protocols for skin biopsies, shaves, and excisions. The histomorphology of skin is reviewed and commonly used H&E and special stains are discussed. Possible sources of problems are also investigated and solutions are applied to prevent artifacts on skin sample slides.

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Histology CE (by LabCE)
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Course provided by LabCE.
Complete skin sections


Elastic stain. Elastic fibers black.


Folds/ tears in tissue


GMS silver fungus black


Grocott's methenamine silver (GMS)


Incomplete and complete skin sections


Mesh Biopsy Cassette


Ribbons from two different blocks on one water bath should be avoided to prevent placing the wrong tissue on the slide.



 
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