Technical Preparation of Bone Marrow Specimens for Histological Assessment (by LabCE)

1 P.A.C.E. contact hour(s)

(based on 62 customer ratings)

Brooke Eguia, MS, BS, HT(ASCP), HTL(ASCP)
Reviewer: Diana Harrington, BS, HT(ASCP)

Course provided by LabCE.

See more courses in: Histology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-030-17, approved through 12/31/2019
Course number 20-628058, approved through 9/1/2020

Objectives

  • Recognize the indications for bone marrow examination.
  • Describe optimal technical preparation techniques for blood smears, bone marrow aspirates, and bone trephine biopsies.
  • Discern common bone marrow preparation components.
  • Understand specialized techniques used for bone marrow evaluation.

Customer Ratings

(based on 62 customer ratings)

Course Outline

  • Clinical Indications
      • Bone Marrow Examination: Clinical Indications
  • Bone Marrow Procurement
      • Bone Marrow Procurement
      • Bone Marrow Procurement: Supplies
      • Bone Marrow Procurement: Aspirate
      • Bone Marrow Procurement: Core Biopsy
      • Aspirate: Optional Immediate Bedside Smear Preparation
      • Core Biopsy: Optional Immediate Bedside Imprint Preparation
  • Bone Marrow Core Imprints
      • Bone Marrow Core Imprints (Touch Preparation)
  • Bone Marrow Aspirate Preparation: Anticoagulated Sample
      • Anticoagulated Aspirate Sample: Bone Marrow Layers
  • Bone Marrow Aspiration: Laboratory Technical Preparation
      • Myeloid-Erythroid (M-E) cells/Concentrate/Buffy Coat Smears
  • Bone Marrow Clot Preparation
      • Bone Marrow Clot Preparation
  • Bone Marrow Core Biopsy
      • Bone Marrow Core Biopsy
  • Iron Stain on Spun Bone Marrow Aspirate Fat-Perivascular Layer
      • Iron Stain on Spun Bone Marrow Aspirate Fat-Perivascular (F-PV) Layer
  • Iron Stain on Bone Marrow Concentrate (Myeloid Erythroid Layer)
      • Technical Preparation of Iron Stain on Bone Marrow Concentrate (Myeloid-Erythroid Layer)
  • Immunohistochemical (IHC) Stains
      • Metastatic Carcinoma Immunohistochemical (IHC) Stains
      • CD138 Immunohistochemical (IHC) Stain
      • CD34 Immunohistochemical (IHC) Stain
      • CD117 or Mast Cell Tryptase Stains
  • Special Stains
      • Reticulin Stain
      • Prussian Blue Iron Stain
      • Bacterial and Fungal Stains
      • Amyloid Stain
  • Histochemical Stains
      • Enzyme Stains
  • Esoteric Testing
      • Esoteric Testing
  • References
      • References

Additional Information

Level of instruction: Basic to intermediate
 
Intended audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.
 
Author information: Brooke Eguia, MS, BS, HT(ASCP), HTL(ASCP) is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology and from Capella University with a Master of Science in Human Service with a specialization in Health Care Administration. During her time as an undergraduate, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College, as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.
 
Reviewer information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. She has also served as a Clinical Education Supervisor at Indiana University and Histotechnology Program Director for Keiser University in Florida.
 
Course description: This course provides information about the clinical indications for bone marrow examination and detailed instruction for the preparation of bone marrow smears used for histological testing. 

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Course provided by LabCE.
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Bone Marrow 1


AD09-173 Bone Marrow Toxoplasma g.
Case of disseminated Toxoplasmosis infection in bone marrow from a heart transplant patient demonstrated with Toxoplasma gondii mouse monoclonal antibody, clone TP3. DAB chromogen.


Image 1


Bone Marrow 21


Reticulin stain on bone marrow core biopsy section



 
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