HIV: Structure, Replication, and Detection (by LabCE)

1.5 P.A.C.E. contact hour(s)

(based on 328 customer ratings)

Author: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB)
Reviewer: Barbara Cebulski, MS, MLS(ASCP)

Course provided by LabCE.

This introductory-level course is designed to meet requirements for HIV/AIDS training, as well as provide basic information about structure and the mechanisms and proteins involved in replication. Appropriate for continuing education for all laboratory personnel. Dr. Pendergraph's extensive experience teaching in the laboratory environment ensures that you get the training you need for your safety and your requirements.

See more courses in: Immunology / Serology

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-066-21, approved through 10/31/2023
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Serology/Immunology): 1.5 hour(s)
Course number 20-858017, approved through 10/31/2023


  • Explain the basic structure of HIV.
  • Discuss the process by which HIV replicates.
  • Discuss commonly used laboratory methods for detection of HIV infection.
  • Compare the advantages and disadvantages of commonly used laboratory test methods for detection of HIV infection.

Customer Ratings

(based on 328 customer ratings)

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Course Outline

  • Structure of the Human Immunodeficiency Virus
      • Basic Structural Components
      • HIV Envelope
      • HIV Capsid
  • Replication
      • Proteins Involved in Adsorption
      • Adsorption
      • Adsorption, continued
      • Penetration and Infection
      • DNA Conversion from RNA
      • HIV is a Retrovirus
      • Infection of the Host Cell
      • Infection of the Host Cell, continued
      • Spread of Infection
      • Spread of Infection, continued
      • Spread of Infection, continued
      • Destruction of the Host Cell
  • HIV Virus Classifications
      • Mutations
  • Progression of HIV Infection
      • Stages of HIV Infection
  • Laboratory Methods to Detect and Monitor HIV Infection
      • HIV Test Methods: Introduction
      • Anti-HIV Immunoassays
      • HIV Immunoassay Methods
      • Rapid Anti-HIV Assays Using Alternate Specimen Types
      • Western Blot
      • Nucleic Acid Testing (NAT)
      • Reverse Transcriptase PCR (RT-PCR)
      • HIV Detection Methods: Comparison Table
      • The Centers for Disease Control and Prevention (CDC) Recommended Algorithm for HIV Testing
  • References
      • References

Additional Information

Level of instruction: Intermediate
Intended audience: Medical laboratory technologists and technicians and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
Author information: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB) received his MSPH from the University of Kentucky in Lexington, his PhD in medical parasitology and mycology from the University of North Carolina in Chapel Hill, and his law degree with a concentration in health care law from Concord Law School, Purdue University. He also did a Fellowship in Tropical Medicine at Louisiana State University School of Medicine. He is the author of a textbook in phlebotomy, a number of scientific articles, plus internet training programs. He is licensed as a laboratory director in the States of Georgia and Florida.
Reviewer information: Barbara Cebulski, MS, MLS(ASCP) has over 40 years of experience in the medical laboratory profession as a technologist, section supervisor, and laboratory manager. She was an Inspection and Technical Specialist for nine years with the College of American Pathologists in the Laboratory Accreditation Program and, until her retirement in 2015, was Program Director for MediaLab, Inc. Barbara holds a Masters in Instructional Technology from Georgia State University.

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Course provided by LabCE.





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