Fundamentals of Molecular Diagnostics (by LabCE)

1 P.A.C.E. contact hour(s)

(based on 727 customer ratings)

Author: Lori Woeste, EdD, MS
Reviewer: Beverly J. Barham, PhD, MPH; Catherine Dragoni, MT(ASCP)SM

Course provided by LabCE.

This course is a comprehensive introduction to the basic principles of the rapidly growing field of molecular diagnostics. Beginning with an overview of essentials and unique terminology, the course addresses many direct and amplified nucleic acid test methods. Specimen handling, and the clinical applications, advantages, and disadvantages of molecular diagnostics are also covered. Most importantly, the principles behind molecular diagnostics are presented in detail, giving you a strong foundation for future exploration and study in molecular diagnostics.

See more courses in: Molecular Pathology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-026-16, approved through 4/30/2018
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Molecular Pathology): 1 hour(s)
Course number 20-547683, approved through 9/1/2018

Objectives

  • Define terms related to molecular diagnostics.
  • Identify appropriate specimen collection and handling measures for molecular diagnostics.
  • Classify the various molecular methodologies.
  • Outline examples of procedures for each molecular diagnostic classification.
  • Describe the principle of each procedure.
  • Discuss clinical applications of molecular diagnostics.

Customer Ratings

(based on 727 customer ratings)

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Course Outline

  • Molecular Diagnostics
      • Overview
      • Overview
      • Targets
      • Basis of Molecular Testing
      • Nucleotides
      • Writing the Code
      • Types of Nucleic Acid Synthesis
      • Human Genome
  • Terms
      • Unique Terminology
      • Terms and Definitions
  • Specimen Collection & Handling
      • Overview
      • Specimen Collection and Handling
      • Pre-analytical Variables
      • Transport
      • Resources
  • Methodology Classifications
      • Classification
      • Classification
  • Classification Specific Procedures
      • Overview
      • Direct Nucleic Acid Testing
      • Amplified Nucleic Acid Testing
  • Procedure Principles
      • Direct Nucleic Acid Testing Principle
      • Direct Nucleic Acid Tests
      • Amplified Nucleic Acid Testing Principle
      • Amplified Nucleic Acid Tests
      • Amplification
      • Thermal Cycling
      • Amplification Issues
      • Hybridization
      • Factors Affecting Hybridization
      • Detection
  • Clinical Applications
      • Overview
      • Why Choose a Molecular Method?
      • Selection
      • When Nucleic Acids Get Altered
      • Infectious Diseases
      • Pharmacogenetics
      • Pharmacogenetics
      • Advantages of Molecular Testing
      • Disadvantages of Molecular Testing
  • References
      • References

Additional Information

Level of instruction: Intermediate

Intended Audience: Clinical laboratory technologists and technicians, and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
 
Author Credentials: Lori Woeste, EdD, MS is an Assistant Professor in the Clinical Laboratory Science Program at Illinois State University in Normal, Illinois. She holds a Masters degree in Health and a Doctorate  in curriculum and instruction. She has taught introductory and advanced courses in clinical chemistry and clinical biochemistry.
 
Reviewer Credentials:  Beverly Barham is an Associate Professor in the Clinical Laboratory Science Program at Illinois State University in Normal, Illinois.  Dr. Barham holds a Masters degree in Public Health and Doctorate in the combined areas of molecular biology, microbiology, and biochemistry.  She has taught introductory and advanced courses in the Clinical Laboratory Science program including courses in immunology, immunohematology, clinical microbiology, parasitology, mycology, and advanced clinical microbiology. 
 
Catherine Dragoni, MT(ASCP)SM received her BS degree in Medical Technology from the State University of New York, Upstate Medical Center, Syracuse.  She began her career as a bench microbiologist at Maine Medical Center, Portland, Maine.  Currently she is the Chief Technologist of Microbiology and Molecular Pathology at NorDx Laboratories, Scarborough, Maine. 
 
Course Description: This course introduces you to the basic principles of molecular diagnostics and the associated terminology. Several direct and amplified nucleic acid test methods are presented and clinical applications are discussed.

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