(based on 66 customer ratings)

Course provided by LabCE.

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Continuing Education Credits

Course number 20-547941, approved through 9/1/2020

Objectives

  • Understand the theoretical concepts used in fluorescence in situ hybridization (FISH) testing.
  • Demonstrate an understanding of the methodologies involved in FISH testing.
  • Acquire an appreciation of the enumeration of fluorescence signals in paraffin-based FISH testing.
  • Identify the equipment necessary to perform FISH testing in a clinical laboratory.

Customer Ratings

(based on 66 customer ratings)

Course Outline

  • Importance of Fluorescence In Situ Hybridization (FISH) Testing in Cancer Care
      • Detecting Genetic Aberrations with Fluorescence In Situ Hybridization (FISH)
      • Drug Therapy Based on Genetic Aberrations
      • DNA Content and Ploidy
      • Genetic Aberrations
      • Traditional Cytogenetics
      • Metaphase Fluorescence In Situ Hybridization (FISH)
      • Interphase Fluorescence In Situ Hybridization (FISH) in Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue
  • Theoretical Knowledge Required for Fluorescence In Situ Hybridization (FISH) Testing
      • Chromosomes
      • Centromeres
      • Probes
      • Centromere Probes
      • Locus-Specific Probes
      • Break-Apart or Translocation Probes
  • Equipment used in FISH technology
      • Equipment Required to Prepare and Store Reagents
      • Equipment Required for Preparation of Tissue
      • Equipment Required for Manual Performance of Fluorescence In Situ Hybridization (FISH) Assay
      • Equipment Required for Analysis of Fluorescence In Situ Hybridization (FISH) Slides
      • Photographing Fluorescence In Situ Hybridization (FISH) Slides
  • FISH methodology
      • Tissue Preparation
      • Common Methodologies
      • Slide Preparation
      • Pre-treatment Strategies
      • Standard Pre-treatment
      • Standard Protease Digestion
      • Standard Denaturation
      • Standard Probe Preparation and Hybridization
      • Standard Post-Hybridization and Completion
      • New Histologic-Based Fluorescence In Situ Hybridization (FISH) Technique
      • Differences in Pre-treatment
      • Differences in Digestion
  • Enumeration of FISH signals
      • Assessing Slide Quality
      • Control Slide
      • Patient Slide
      • HER2/neu Examples
      • Reporting Fluorescence In Situ Hybridization (FISH)
      • International Standing Committee on Cytogenetic Nomenclature (ISCN) Reporting
  • References
      • References
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Course provided by LabCE.

 
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