Tissue Processing in Histology (by LabCE)

(based on 241 customer ratings)

Author: Diana Harrington, BS, HT(ASCP)
Reviewer: Brooke Eguia, BS, HT(ASCP), HTL(ASCP)

Course provided by LabCE.

This purpose of this course is to discuss the appropriate application of the most common tissue processing steps utilized in today's histology laboratory and provide troubleshooting guidelines for tissue that has been improperly processed. The various factors affecting tissue processing will also be explored, including comparisons of different tissue types and their required processing cycles.

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Continuing Education Credits

Course number 20-547959, approved through 9/1/2020

Objectives

  • Describe the steps involved in processing tissue and the various reagents used in the process.
  • Identify the most common reagents used in conventional tissue processing and their effect on tissue.
  • Identify the various factors affecting processing and compare how different tissue types require different processing cycles.
  • Relate how quality control measures ensure consistent tissue processing and identify tissue that is poorly processed and state a remedy for the problem.
  • Identify the hazards associated with the most common processing reagents.

Customer Ratings

(based on 241 customer ratings)

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Course Outline

  • Reagent Steps of Tissue Processing
      • Processing Steps Defined
  • Conventional Processing - In Depth
      • Tissue Fixation
      • Formalin Fixative
      • Tissue Dehydration
      • Tissue Clearing
      • Paraffin Infiltration
      • Xylene Free Processing
  • Processing Equipment and Factors Affecting Tissue Processing
      • Processing Equipment
      • Automated Tissue Processors
      • Factors Affecting Tissue Processing
      • Time Factor
      • Temperature Factor
      • Pressure Factor
      • Standard Processing Protocols
      • Routine Tissue Processing
      • Biopsy Tissue Processing
      • Fatty Tissue Processing
      • Special Tissue Processing
  • Processing Quality Control Measures
      • Reagent Maintenance and Temperature Checks
      • Troubleshooting Processing Problems
  • Reagent Safety
      • Reagent Safety
      • Formalin and Xylene Monitoring
  • References
      • References

Additional Information

Level of instruction: Basic
 
Intended audience:  Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.  
 
Author information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
 
Reviewer information: Brooke Eguia, BS, HT(ASCP), HTL(ASCP) is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology. While in college, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. As a senior in college, she co-authored for aquatic toxicology research that Aquaculture published in January 2009. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College, as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.   
 
Course description:  This purpose of this course is to discuss the appropriate application of the most common tissue processing steps utilized in today's histology laboratory and provide troubleshooting guidelines for tissue that has been improperly processed. The various factors affecting tissue processing will also be explored, including comparisons of different tissue types and their required processing cycles. 

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Histology CE (by LabCE)
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Course provided by LabCE.
Alcian blue 2.5 new


Factors affecting tissue processing


Formaldehyde monitors


Open tissue processor. Image courtesy of Wikimedia Commons.


Specimens 400x


Paraffin Embedded Tissue Block


Vacuum tissue processor


Properly processed fatty tissue ribbon floating on water bath.



 
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