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Continuing Education Credits

Florida Board of Clinical Laboratory Personnel Credit Hours - Immunohistochemistry/Advanced Histologic Techniques: 2 hour(s)
Course number 20-547715, approved through 9/1/2020


  • Identify infectious entities on formalin-fixed, paraffin-embedded human tissue by use of specific primary antibodies and immunohistochemistry (IHC) techniques.
  • Describe specific entities that cause various infectious diseases.
  • Discuss both monoclonal and polyclonal antibodies that are available for use in the detection of infectious diseases.
  • Outline IHC techniques, including the use of alternative methodologies, chromogenic substrates, and enhancement techniques.

Customer Ratings

(based on 96 customer ratings)

Course Outline

  • Introduction
      • Using Immunohistochemistry (IHC) for the Detection of Infectious Diseases
      • Disclaimer
      • IHC Detection Systems
      • Tissue Pretreatment: Heat-Induced Epitope Retrieval (HIER)
      • Heat-Induced Epitope Retrieval (HIER) Equipment
      • Tissue Pretreatment: Enzyme-Induced Epitope Retrieval (EIER)
      • Tissue Adhesion
      • Enzyme Labels
      • Chromogenic Substrates
  • Infectious Disease Entities: Antibodies and Reagents used in Immunohistochemistry (IHC)
      • Antibodies
      • Viral Markers
    • Cytomegalovirus (CMV)
      • Cytomegalovirus (CMV)
      • Additional Cytomegalovirus (CMV) Images
    • Herpes Simplex Virus (HSV)
      • Herpes Simplex Virus (HSV)
      • Additional Herpes Simplex Virus (HSV) Images
    • Adenovirus (ADV)
      • Adenovirus (ADV)
      • Additional Adenovirus (ADV) Image
    • Epstein-Barr Virus (EBV)
      • Epstein-Barr Virus (EBV)
      • Epstein-Barr Virus (EBV) Latent Membrane Protein 1 (LMP1)
      • Epstein-Barr Virus (EBV) Epstein-Barr Nuclear Antigen 2 (EBNA2)
    • Varicella Zoster Virus (VZV)
      • Varicella Zoster Virus (VZV)
    • Human Papilloma Virus (HPV)
      • Human Papilloma Virus (HPV)
    • Polyomavirus
      • Polyomavirus
      • JC Polyomavirus (JCV)
      • BK Polyomavirus (BKV)
    • Hepatitis B Virus
      • Hepatitis B Virus (HBV)
      • Hepatitis B Core Antigen (HBcAg)
      • Hepatitis B Surface Antigen (HBsAg)
      • IHC Double Stain for Hepatitis B Surface Antigen (HBsAg) and Hepatitis B Core Antigen (HBcAg)
    • Parvovirus
      • Parvovirus (PARVO) B19
    • Parainfluenza
      • Human Parainfluenza Virus (HPIV)
    • Respiratory scintial virus (RSV)
      • Respiratory Syncytial Virus (RSV)
    • Human Herpes Virus 8 (HHV8)
      • Human Herpesvirus 8 (HHV8)
  • Parasitic or Fungal Entities: Antibodies and Reagents used in Immunohistochemistry (IHC)
      • Pneumocystis Pneumonia (PCP) or Pneumocystosis
      • Toxoplasmosis
      • Additional Images of Toxoplasmosis
  • Bacterial Entities: Antibodies and Reagents used in IHC
    • Treponema pallidum
      • Treponema pallidum
    • Helicobacter pylori
      • Helicobacter pylori
      • Addtional Images of Helicobacter pylori
  • References
      • References


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