Immunohistochemistry (IHC) - Detection and Identification of Infectious Disease Processes in Surgical Pathology (by LabCE)

(based on 98 customer ratings)

Author: Jim Burchette, HT(ASCP)
Reviewer: M. Lamar Jones, BS, HT(ASCP)

Course provided by LabCE.

This course discusses various entities that cause infectious diseases. These entities are identified in formalin-fixed, paraffin-embedded (FFPE) human tissue by use of specific primary antibodies and immunohistochemical (IHC) techniques. IHC procedures, including chromogenic substrates, enhancement techniques, and alternative methodologies are also discussed.

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Continuing Education Credits

Florida Board of Clinical Laboratory Personnel Credit Hours - Immunohistochemistry/Advanced Histologic Techniques: 2 hour(s)
Course number 20-547715, approved through 9/1/2020

Objectives

  • Identify infectious entities on formalin-fixed, paraffin-embedded human tissue by use of specific primary antibodies and immunohistochemistry (IHC) techniques.
  • Describe specific entities that cause various infectious diseases.
  • Discuss both monoclonal and polyclonal antibodies that are available for use in the detection of infectious diseases.
  • Outline IHC techniques, including the use of alternative methodologies, chromogenic substrates, and enhancement techniques.

Customer Ratings

(based on 98 customer ratings)

Course Outline

  • Introduction
      • Using Immunohistochemistry (IHC) for the Detection of Infectious Diseases
      • Disclaimer
      • IHC Detection Systems
      • Tissue Pretreatment: Heat-Induced Epitope Retrieval (HIER)
      • Heat-Induced Epitope Retrieval (HIER) Equipment
      • Tissue Pretreatment: Enzyme-Induced Epitope Retrieval (EIER)
      • Tissue Adhesion
      • Enzyme Labels
      • Chromogenic Substrates
  • Infectious Disease Entities: Antibodies and Reagents used in Immunohistochemistry (IHC)
      • Antibodies
      • Viral Markers
    • Cytomegalovirus (CMV)
      • Cytomegalovirus (CMV)
      • Additional Cytomegalovirus (CMV) Images
    • Herpes Simplex Virus (HSV)
      • Herpes Simplex Virus (HSV)
      • Additional Herpes Simplex Virus (HSV) Images
    • Adenovirus (ADV)
      • Adenovirus (ADV)
      • Additional Adenovirus (ADV) Image
    • Epstein-Barr Virus (EBV)
      • Epstein-Barr Virus (EBV)
      • Epstein-Barr Virus (EBV) Latent Membrane Protein 1 (LMP1)
      • Epstein-Barr Virus (EBV) Epstein-Barr Nuclear Antigen 2 (EBNA2)
    • Varicella Zoster Virus (VZV)
      • Varicella Zoster Virus (VZV)
    • Human Papilloma Virus (HPV)
      • Human Papilloma Virus (HPV)
    • Polyomavirus
      • Polyomavirus
      • JC Polyomavirus (JCV)
      • BK Polyomavirus (BKV)
    • Hepatitis B Virus
      • Hepatitis B Virus (HBV)
      • Hepatitis B Core Antigen (HBcAg)
      • Hepatitis B Surface Antigen (HBsAg)
      • IHC Double Stain for Hepatitis B Surface Antigen (HBsAg) and Hepatitis B Core Antigen (HBcAg)
    • Parvovirus
      • Parvovirus (PARVO) B19
    • Parainfluenza
      • Human Parainfluenza Virus (HPIV)
    • Respiratory scintial virus (RSV)
      • Respiratory Syncytial Virus (RSV)
    • Human Herpes Virus 8 (HHV8)
      • Human Herpesvirus 8 (HHV8)
  • Parasitic or Fungal Entities: Antibodies and Reagents used in Immunohistochemistry (IHC)
      • Pneumocystis Pneumonia (PCP) or Pneumocystosis
      • Toxoplasmosis
      • Additional Images of Toxoplasmosis
  • Bacterial Entities: Antibodies and Reagents used in IHC
    • Treponema pallidum
      • Treponema pallidum
    • Helicobacter pylori
      • Helicobacter pylori
      • Addtional Images of Helicobacter pylori
  • References
      • References

Additional Information

Level of instruction: Intermediate to advanced
 
Intended audience:  This program is designed  for the clinical immunohistochemist that has a working knowledge of immunohistochemistry (IHC) technique and can also be used as a reference for proper staining patterns and morphology. This course is also appropriate as an educational and training tool for histology laboratory personnel. 
 
Author information: Jim Burchette, HT(ASCP) recently retired after 34 years at Duke University Medical Center, Durham, North Carolina. As an Immunopathology Analytical Specialist in Molecular Pathology, Jim's primary job functions included daily IHC quality control and managing the daily technical aspect of the Clinical Immunopathology operation. He has over 38 years of experience in surgical pathology histotechnology with 33 years of immunopathology specialty. Jim has an extensive experience validating new antibodies for research and diagnostic immunohistochemical use. To date, Jim has 80 publications (70 of which are peer-reviewed) and two book chapters, as well as many abstracts, posters, and technical acknowledgments in peer reviewed scientific articles. He has also served as a consultant for Triangle Biomedical Sciences (TBS), Dako North America, Leica Microsystems, and Polysciences.
 
Reviewer information:  Lamar Jones, BS, HT(ASCP) holds a BS degree from the University of Kentucky, Lexington, Kentucky. He received histotechnology training from the School of Histotechnology at the University of Tennessee Medical Units, Memphis, Tennessee. Lamar is currently the Technical Coordinator in the Department of Pathology at Emory University Hospital in Atlanta, Georgia. He also serves as the HT Program Director for the School of Histotechnology at Davidson County Community College, Mocksville, North Carolina and serves as lecturer, author, workshop director, and presenter for local, state, and national histotechnology meetings. 
  
Course description:  This course discusses various entities that cause infectious diseases. These entities are identified in formalin-fixed, paraffin-embedded (FFPE) human tissue by use of specific primary antibodies and immunohistochemical (IHC) techniques. IHC procedures, including chromogenic substrates, enhancement techniques, and alternative methodologies are also discussed.

Keywords

These are the most common topics and keywords covered in Immunohistochemistry (IHC) - Detection and Identification of Infectious Disease Processes in Surgical Pathology (by LabCE):

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AD09-173 Bone Marrow Toxoplasma g.
Case of disseminated Toxoplasmosis infection in bone marrow from a heart transplant patient demonstrated with Toxoplasma gondii mouse monoclonal antibody, clone TP3. DAB chromogen.


EBV positive high grade malignant lymphoma immunoblastic plasmacytoid type. Nuclear reaction shown with EBV EBNA2 antibody. DAB chromogen.


Helicobacter pylori bacteria demonstrated in gastrointestinal tissue using rabbit polyclonal antibody HRP labeled detection system with DAB chromogen.


Toxoplasmosis infection in lung demonstrated with Toxoplasma gondii mouse monoclonal antibody, clone TP3. Fast red chromogen. Notice the clusters of T gondii protozoa and individual protoza. Tissue cysts will rupture, releasing individual protoza to form new cysts.


Virus-like particles in the nuclei of abnormal oligodendrocytes are labeling with mouse monoclonal SV40 antibody, clone MRQ-4. DAB chromogen.


CMV image A


Treponema pallidum (spirochetes) demonstrated in a cutaneous biopsy from an HIV patient with epidermal ulcerations and dermal lymphohistiocytic inflammation. Fast red chromogen.


Pretreatment (PT) module



 
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