Vitamin D (by LabCE)

1 P.A.C.E. contact hour(s)

(based on 1,401 customer ratings)

Author: Lynne Brodeur, MA, MLS(ASCP)CM
Reviewers: Kevin F. Foley, PhD, DABCC, MT, SC; Krista Moore, MLS(ASCP)CM

Course provided by LabCE.

This course discusses the role of Vitamin D in the human body and helps differentiate between the various forms of vitamin D. The mechanisms of metabolism of vitamin D are presented along with the role of the laboratory in the testing for vitamin D. The analytical variables that may affect vitamin D testing are also discussed in this course.

See more courses in: Clinical Chemistry / Urinalysis / Toxicology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-116-20, approved through 12/31/2022
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 1 hour(s)
Course number 20-804158, approved through 12/31/2022


  • Differentiate between the various forms of vitamin D.
  • Explain the mechanisms of metabolism of vitamin D.
  • Discuss vitamin D deficiency, screening, and risk factors.
  • List the various methodologies for testing vitamin D.
  • List the analytical variables that may affect vitamin D testing.

Customer Ratings

(based on 1,401 customer ratings)

Course Outline

  • The Different Forms of Vitamin D
      • Role of Vitamin D
      • Receptors
      • Vitamin D Forms
      • Vitamin D2 Form
      • Vitamin D3 Form
  • Vitamin D Sources and Metabolism
      • Sources
      • Metabolism
      • Absorption and Excretion
      • Recommended Daily Intake of Vitamin D
  • Causes of Vitamin D Deficiency
      • Decreased Intake
      • Sunlight and Skin Pigmentation
      • Sunscreen
      • Age
      • Absorption Issues
      • Renal Disease
  • Diseases Associated with Decreased Vitamin D
      • Children (Growth and Development)
      • Hyperparathyroidism
      • Osteoporosis and Osteopenia
      • Immune System Impairment
      • Rickets
  • Vitamin D Testing Methods and Methodologies
      • Historical Measurements
      • The Reference Method
      • HPLC
      • Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
      • Immunoassays
      • Sample Requirements
  • Interferences
      • Medications
      • Antibody and Other Interferences
  • References
      • References

Additional Information

Level of Instruction: Intermediate
Intended Audience: Medical laboratory scientists, medical laboratory technicians, pathology residents, MLS students, and other healthcare personnel who have an interest in this subject matter.
Author Information: Lynne Brodeur, MA, MLS(ASCP)CM holds a master of arts degree in teaching. She is currently a full time lecturer at the University of Massachusetts Dartmouth and works per diem in Clinical Chemistry at St. Luke's Hospital in New Bedford, MA.
Reviewer Information:
Kevin F. Foley, PhD, DABCC, MT, SC is the director of clinical pathology for the Kaiser Permanente Northwest region. He also teaches clinical chemistry at Oregon Health Sciences University. Dr. Foley earned his PhD in clinical pharmacology and toxicology at East Carolina School of Medicine in North Carolina.
Krista M. Moore, MLS(ASCP)CM is a Technical Specialist for Chemistry, Immunology, and Toxicology at Kaiser Permanente, Portland, Oregon. She holds BS degrees in Clinical Laboratory Science from Oregon Health Science University/Oregon Institute of Technology and in Chemistry from State University of NY at Geneseo. 

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Course provided by LabCE.
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