Histology Special Stains: Connective Tissue (by LabCE)

1 P.A.C.E. contact hour(s)

(based on 112 customer ratings)

Author: Tanisha N. Neely, HT(ASCP)
Reviewer: Diana Harrington, BS, HT(ASCP)

Course provided by LabCE.

This course is the second in a series of courses that will explore special stains used in the clinical histology laboratory. Basic connective tissue staining mechanisms and classifying connective tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate connective tissue in samples.

See more courses in: Histology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-067-16, approved through 7/31/2018
Course number 20-547699, approved through 9/1/2018

Objectives

  • Understand basic tissue staining methods used in the clinical histology laboratory.
  • Explain factors that affect dye binding and utilize to troubleshoot staining issues.
  • Classify connective tissue elements and identify which dyes will bind to these elements.
  • Demonstrate safe microwave use when necessary to perform special staining procedures.

Customer Ratings

(based on 112 customer ratings)

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Course Outline

  • Introduction
      • Tissue Staining in the Clinical Histology Laboratory
      • The Biological Stain Commission (BSC)
  • Basic Staining Mechanisms
      • What Makes a Dye?
      • Factors That Affect Dye Binding
      • Acids, Bases, pH, and Buffers
      • Basophilic and Acidophilic Staining
      • Oxidation and Reduction
  • Connective Tissue Classification and Functions
      • What is Connective Tissue?
      • Classifying Connective Tissue
      • Functions of Connective Tissue
  • Special Staining Methods Used to Demonstrate Connective Tissue
      • Gordon and Sweet's Silver Staining - Chemistry
      • Gordon and Sweet's Silver Staining - Diagnostic Applications
      • Gordon and Sweet's Silver Staining - Staining Protocol
      • Verhoeff-Van Gieson (VVG) Staining - Chemistry
      • Verhoeff-Van Gieson (VVG) Staining - Diagnostic Applications
      • Verhoeff-Van Gieson (VVG) Staining - Staining Protocol
      • Toluidine Blue Staining - Chemistry
      • Toluidine Blue Staining - Diagnostic Applications
      • Toluidine Blue Staining - Staining Protocol
      • Masson's Trichrome Staining - Chemistry
      • Masson's Trichrome Staining - Diagnostic Applications
      • Masson's Trichrome Staining - Staining Protocol
      • Phosphotungstic Acid-Hematoxylin (PTAH) Staining - Chemistry
      • Phosphotungstic Acid-Hematoxylin (PTAH) Staining - Diagnostic Applications
      • Phosphotungstic Acid-Hematoxylin (PTAH) Staining - Staining Protocol
  • Using the Microwave for Special Staining Procedures
      • Microwaves in the Histopathology Laboratory
      • Tips for using the Microwave for Special Staining Procedures
      • Safety Precautions for Microwave Usage
  • References
      • References

Additional Information

Level of instruction: Intermediate
 
Intended audience:  Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.  
 
Author information: Tanisha N. Neely, HT(ASCP) is a freelance writer with a work history in histology, microbiology, and pharmaceutical research. Ms. Neely attended Indiana University where she earned a bachelors degree in arts & humanities from the School of General Studies and a Certificate in Histotechnology from the School of Medicine. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
 
Reviewer information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
 
Course description: This course is the second in a series of courses that will explore special stains used in the clinical histology laboratory. Basic connective tissue staining mechanisms and classifying connective tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate connective tissue in samples. 

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Histology CE (by LabCE)
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Course provided by LabCE.
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